PTP4A2 Antibody

NSJ Bioreagents
Product Code: NSJ-F54431
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54431-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54431-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PTP4A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human NCI-H460 cell lysate with PTP4A2 antibody. Predicted molecular weight ~19 kDa.
2 / 5
Western blot testing of human HeLa cell lysate with PTP4A2 antibody. Predicted molecular weight ~19 kDa.
3 / 5
Immunofluorescent staining of human prostate carcinoma cells with PTP4A2 antibody (green) PI (red).
4 / 5
IHC testing of FFPE human prostate carcinoma tissue with PTP4A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human WiDr cells with PTP4A2 antibody; Blue=isotype control, Green= PTP4A2 antibody.

Western blot testing of human NCI-H460 cell lysate with PTP4A2 antibody. Predicted molecular weight ~19 kDa.
Western blot testing of human HeLa cell lysate with PTP4A2 antibody. Predicted molecular weight ~19 kDa.
Immunofluorescent staining of human prostate carcinoma cells with PTP4A2 antibody (green) PI (red).
IHC testing of FFPE human prostate carcinoma tissue with PTP4A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human WiDr cells with PTP4A2 antibody; Blue=isotype control, Green= PTP4A2 antibody.

Further Information

Application Details :
Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PTP4A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PTP4A2 belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 32-59 from the human protein was used as the immunogen for the PTP4A2 antibody.
Limitation:
This PTP4A2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q12974