NR1I2 Antibody / PXR

Contact us
NSJ Bioreagents
Product Code: NSJ-F54461
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54461-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54461-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PXR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human HL60 cell lysate with PXR antibody. Predicted molecular weight ~50 kDa.
2 / 5
Western blot testing of human HeLa cell lysate with PXR antibody. Predicted molecular weight ~50 kDa.
3 / 5
IHC testing of FFPE human hepatocarcinoma tissue with PXR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with PXR antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
5 / 5
Flow cytometry testing of human HL60 cells with PXR antibody; Blue=isotype control, Green= PXR antibody.
❮❯

Western blot testing of human HL60 cell lysate with PXR antibody. Predicted molecular weight ~50 kDa.
Western blot testing of human HeLa cell lysate with PXR antibody. Predicted molecular weight ~50 kDa.
IHC testing of FFPE human hepatocarcinoma tissue with PXR antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with PXR antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of human HL60 cells with PXR antibody; Blue=isotype control, Green= PXR antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PXR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NR1I2/PXR belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. This protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 100-127 from the human protein was used as the immunogen for the PXR antibody.
Limitation:
This PXR antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O75469