SSH3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54610
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54610-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54610-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Slingshot homolog 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human breast carcinoma tissue with Slingshot homolog 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HeLa cells with Slingshot homolog 3 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
Western blot testing of human ZR-75-1 cell lysate with Slingshot homolog 3 antibody. Predicted molecular weight ~73 kDa, can be observed at 90-95 kDa.
4 / 4
Flow cytometry testing of human HeLa cells with Slingshot homolog 3 antibody; Blue=isotype control, Green= Slingshot homolog 3 antibody.
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IHC testing of FFPE human breast carcinoma tissue with Slingshot homolog 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Slingshot homolog 3 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of human ZR-75-1 cell lysate with Slingshot homolog 3 antibody. Predicted molecular weight ~73 kDa, can be observed at 90-95 kDa.
Flow cytometry testing of human HeLa cells with Slingshot homolog 3 antibody; Blue=isotype control, Green= Slingshot homolog 3 antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Slingshot homolog 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The ADF (actin-depolymerizing factor)/cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 575-602 from the human protein was used as the immunogen for the Slingshot homolog 3 antibody.
Limitation:
This Slingshot homolog 3 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q8TE77