TIMP1 Antibody

NSJ Bioreagents
Product Code: NSJ-F54876
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54876-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54876-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TIMP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human CCRF-CEM cell lysate with TIMP1 antibody. Predicted molecular weight ~23 kDa.
2 / 4
IHC testing of FFPE human breast carcinoma tissue with TIMP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human A2058 cells with TIMP1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of fixed and permeabilized human MDA-MB-231 cells with TIMP1 antibody; Blue=isotype control, Green= TIMP1 antibody.

Western blot testing of human CCRF-CEM cell lysate with TIMP1 antibody. Predicted molecular weight ~23 kDa.
IHC testing of FFPE human breast carcinoma tissue with TIMP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human A2058 cells with TIMP1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human MDA-MB-231 cells with TIMP1 antibody; Blue=isotype control, Green= TIMP1 antibody.

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the TIMP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TIMP1 belongs to the TIMP family. The proteins in this family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 41-70 from the human protein was used as the immunogen for the TIMP1 antibody.
Limitation:
This TIMP1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P01033