TNFR1-associated DEATH domain protein Antibody / TRADD

Contact us
NSJ Bioreagents
Product Code: NSJ-F54628
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54628-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54628-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TRADD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human breast carcinoma tissue with TRADD antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HeLa cells with TRADD antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
Western blot testing of human HeLa cell lysate with TRADD antibody. Predicted molecular weight ~34 kDa.
4 / 4
Flow cytometry testing of human HeLa cells with TRADD antibody; Blue=isotype control, Green= TRADD antibody.
❮❯

IHC testing of FFPE human breast carcinoma tissue with TRADD antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with TRADD antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of human HeLa cell lysate with TRADD antibody. Predicted molecular weight ~34 kDa.
Flow cytometry testing of human HeLa cells with TRADD antibody; Blue=isotype control, Green= TRADD antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the TRADD antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 126-151 from the human protein was used as the immunogen for the TRADD antibody.
Limitation:
This TNFR1-associated DEATH domain protein antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q15628