UDP-glucose 4-epimerase Antibody / GALE

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NSJ Bioreagents
Product Code: NSJ-F54898
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54898-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54898-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the UDP-glucose 4-epimerase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human skin tissue with UDP-glucose 4-epimerase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human WiDr cells with UDP-glucose 4-epimerase antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human A375 cell lysate with UDP-glucose 4-epimerase antibody. Predicted molecular weight ~38 kDa.
4 / 4
Flow cytometry testing of human HeLa cells with UDP-glucose 4-epimerase antibody; Blue=isotype control, Green= UDP-glucose 4-epimerase antibody.
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IHC testing of FFPE human skin tissue with UDP-glucose 4-epimerase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human WiDr cells with UDP-glucose 4-epimerase antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human A375 cell lysate with UDP-glucose 4-epimerase antibody. Predicted molecular weight ~38 kDa.
Flow cytometry testing of human HeLa cells with UDP-glucose 4-epimerase antibody; Blue=isotype control, Green= UDP-glucose 4-epimerase antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the UDP-glucose 4-epimerase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
UDP-galactose-4-epimerase catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 142-171 from the human protein was used as the immunogen for the UDP-glucose 4-epimerase antibody.
Limitation:
This UDP-glucose 4-epimerase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q14376