VPS52 Antibody / SACM2L

NSJ Bioreagents
Product Code: NSJ-F54565
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54565-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54565-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the VPS52 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human MDA-MB-435 cell lysate with VPS52 antibody. Predicted molecular weight ~82 kDa (isoform 1) and 68 kDa (isoform 2).
2 / 4
IHC testing of FFPE human colon carcinoma tissue with VPS52 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human MCF7 cells with VPS52 antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human MDA-MB-435 cells with VPS52 antibody; Blue=isotype control, Green= VPS52 antibody.

Western blot testing of human MDA-MB-435 cell lysate with VPS52 antibody. Predicted molecular weight ~82 kDa (isoform 1) and 68 kDa (isoform 2).
IHC testing of FFPE human colon carcinoma tissue with VPS52 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MCF7 cells with VPS52 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human MDA-MB-435 cells with VPS52 antibody; Blue=isotype control, Green= VPS52 antibody.

Further Information

Application Details :
Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the VPS52 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 610-637 from the human protein was used as the immunogen for the VPS52 antibody.
Limitation:
This VPS52 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q8N1B4