SOX2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54738
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54738-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54738-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the SOX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human NCCIT cell lysate with SOX2 antibody. Predicted molecular weight ~34 kDa.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with SOX2 antibody.
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Immunofluorescent staining of fixed and permeabilized human SH-SY5Y cells with SOX2 antibody (green) and anti-Actin (red).
4 / 4
Flow cytometry testing of human HEK293 cells with SOX2 antibody; Blue=isotype control, Green= SOX2 antibody.
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Western blot testing of human NCCIT cell lysate with SOX2 antibody. Predicted molecular weight ~34 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with SOX2 antibody.
Immunofluorescent staining of fixed and permeabilized human SH-SY5Y cells with SOX2 antibody (green) and anti-Actin (red).
Flow cytometry testing of human HEK293 cells with SOX2 antibody; Blue=isotype control, Green= SOX2 antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25, Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 251-284 from the human protein was used as the immunogen for the SOX2 antibody.
Limitation:
This SOX2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P48431