Aspa Antibody / Aspartoacylase

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NSJ Bioreagents
Product Code: NSJ-F54432
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54432-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54432-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Aspartoacylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human skeletal muscle tissue with Aspartoacylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with Aspartoacylase antibody (green) and DAPI nuclear stain (blue).
3 / 5
Western blot testing of human MCF7 cell lysate with Aspartoacylase antibody. Predicted molecular weight ~36 kDa.
4 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Aspartoacylase antibody.
5 / 5
Flow cytometry testing of human MDA-MB-468 cells with Aspartoacylase antibody; Blue=isotype control, Green= Aspartoacylase antibody.
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IHC testing of FFPE human skeletal muscle tissue with Aspartoacylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with Aspartoacylase antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human MCF7 cell lysate with Aspartoacylase antibody. Predicted molecular weight ~36 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Aspartoacylase antibody.
Flow cytometry testing of human MDA-MB-468 cells with Aspartoacylase antibody; Blue=isotype control, Green= Aspartoacylase antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Aspartoacylase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ASPA is an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 82-110 from the human protein was used as the immunogen for the Aspartoacylase antibody.
Limitation:
This Aspartoacylase antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
P45381