ESRRA Antibody / Estrogen-Related Receptor Alpha

NSJ Bioreagents
Product Code: NSJ-F54460
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54460-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54460-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Estrogen-Related Receptor Alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human A375 cell lysate with Estrogen-Related Receptor Alpha antibody. Expected molecular weight: 46-52 kDa.
2 / 4
IHC testing of FFPE human kidney carcinoma tissue with Estrogen-Related Receptor Alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human HEK293 cells with Estrogen-Related Receptor Alpha antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human HEK293 cells with Estrogen-Related Receptor Alpha antibody; Blue=isotype control, Green= Estrogen-Related Receptor Alpha antibody.

Western blot testing of human A375 cell lysate with Estrogen-Related Receptor Alpha antibody. Expected molecular weight: 46-52 kDa.
IHC testing of FFPE human kidney carcinoma tissue with Estrogen-Related Receptor Alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HEK293 cells with Estrogen-Related Receptor Alpha antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human HEK293 cells with Estrogen-Related Receptor Alpha antibody; Blue=isotype control, Green= Estrogen-Related Receptor Alpha antibody.

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Estrogen-Related Receptor Alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ESRRA is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 131-159 from the human protein was used as the immunogen for the Estrogen-Related Receptor Alpha antibody.
Limitation:
This Estrogen-Related Receptor Alpha antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P11474