GTF2I Antibody / TFII-I

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NSJ Bioreagents
Product Code: NSJ-F54433
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54433-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54433-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the GTF2I antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Immunofluorescent staining of fixed and permeabilized human A549 cells with GTF2I antibody (green) and anti-Actin (red).
2 / 5
Immunofluorescent staining of human HeLa cells with GTF2I antibody (green) and anti-Actin (red).
3 / 5
IHC testing of FFPE human brain tissue with GTF2I antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
Western blot testing of human 1) A431 and 2) HeLa lysate with GTF2I antibody. Predicted molecular weight ~112 kDa but can be observed at up to ~135 kDa.
5 / 5
Flow cytometry testing of human K562 cells with GTF2I antibody; Blue=isotype control, Green= GTF2I antibody.
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Immunofluorescent staining of fixed and permeabilized human A549 cells with GTF2I antibody (green) and anti-Actin (red).
Immunofluorescent staining of human HeLa cells with GTF2I antibody (green) and anti-Actin (red).
IHC testing of FFPE human brain tissue with GTF2I antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) A431 and 2) HeLa lysate with GTF2I antibody. Predicted molecular weight ~112 kDa but can be observed at up to ~135 kDa.
Flow cytometry testing of human K562 cells with GTF2I antibody; Blue=isotype control, Green= GTF2I antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the GTF2I antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
GTF2I is a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 956-985 from the human protein was used as the immunogen for the GTF2I antibody.
Limitation:
This GTF2I antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P78347