OTU1 Antibody / YOD1 / DUBA-8

NSJ Bioreagents
Product Code: NSJ-F54592
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54592-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54592-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the OTU1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western bot testing of two different lots of human K562 cell lysate with OTU1 antibody. Predicted molecular weight ~38 kDa.
2 / 3
IHC testing of FFPE human colon tissue with OTU1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
Immunofluorescent staining of human WiDr cells with OTU1 antibody (green) and DAPI nuclear stain (blue).

Western bot testing of two different lots of human K562 cell lysate with OTU1 antibody. Predicted molecular weight ~38 kDa.
IHC testing of FFPE human colon tissue with OTU1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human WiDr cells with OTU1 antibody (green) and DAPI nuclear stain (blue).

Further Information

Application Details :
Immunofluorescence: 1:25, Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the OTU1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Deubiquitinating enzymes (DUBS) are proteases that specifically cleave ubiquitin linkages, negating the action of ubiquitin ligases. DUBA-8 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain. [OMIM].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 319-347 from the human protein was used as the immunogen for the OTU1 antibody.
Limitation:
This OTU1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q5VVQ6