ALOX15 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54516
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54516-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54516-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ALOX15 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human lymph tissue with ALOX15 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Immunofluorescent staining of human HeLa cells with ALOX15 antibody (green) and DAPI nuclear stain (blue).
3 / 3
Western blot testing of human 1) HeLa, 2) A549, 3) K562 and 4) MDA-MB-453 cell lysate with ALOX15 antibody. Predicted molecular weight ~75 kDa.
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IHC testing of FFPE human lymph tissue with ALOX15 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with ALOX15 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human 1) HeLa, 2) A549, 3) K562 and 4) MDA-MB-453 cell lysate with ALOX15 antibody. Predicted molecular weight ~75 kDa.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the ALOX15 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 610-643 from the human protein was used as the immunogen for the ALOX15 antibody.
Limitation:
This ALOX15 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P16050