Beta-TrCP Antibody / BTRC

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NSJ Bioreagents
Product Code: NSJ-F54622
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54622-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54622-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Beta-TrCP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) HepG2 and 2) HEK293 cell lysate with Beta-TrCP antibody. Predicted molecular weight ~69 kDa.
2 / 4
Western blot testing of human ZR-75-1 cell lysate with Beta-TrCP antibody. Predicted molecular weight ~69 kDa.
3 / 4
Immunofluorescent staining of human ZR-75-1 cells with Beta-TrCP antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
IHC testing of FFPE human brain tissue with Beta-TrCP antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HepG2 and 2) HEK293 cell lysate with Beta-TrCP antibody. Predicted molecular weight ~69 kDa.
Western blot testing of human ZR-75-1 cell lysate with Beta-TrCP antibody. Predicted molecular weight ~69 kDa.
Immunofluorescent staining of human ZR-75-1 cells with Beta-TrCP antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human brain tissue with Beta-TrCP antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Beta-TrCP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 17-52 from the human protein was used as the immunogen for the Beta-TrCP antibody.
Limitation:
This Beta-TrCP antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9Y297