CalDAG-GEFI Antibody / RASGRP2

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NSJ Bioreagents
Product Code: NSJ-F54641
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54641-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54641-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CalDAG-GEFI antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human spleen tissue lysate with CalDAG-GEFI antibody. Predicted molecular weight ~69 kDa.
2 / 4
Western blot testing of human K562 cell lysate with CalDAG-GEFI antibody. Predicted molecular weight ~69 kDa.
3 / 4
Immunofluorescent staining of U-251 MG cells with CalDAG-GEFI antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
IHC testing of FFPE human brain tissue with CalDAG-GEFI antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human spleen tissue lysate with CalDAG-GEFI antibody. Predicted molecular weight ~69 kDa.
Western blot testing of human K562 cell lysate with CalDAG-GEFI antibody. Predicted molecular weight ~69 kDa.
Immunofluorescent staining of U-251 MG cells with CalDAG-GEFI antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human brain tissue with CalDAG-GEFI antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the CalDAG-GEFI antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 7-34 from the human protein was used as the immunogen for the CalDAG-GEFI antibody.
Limitation:
This CalDAG-GEFI antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q7LDG7