Corticotropin-releasing factor receptor 2 Antibody / CRHR2

NSJ Bioreagents
Product Code: NSJ-F54883
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54883-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54883-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Corticotropin-releasing factor receptor 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE mouse brain tissue with Corticotropin-releasing factor receptor 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HeLa cells with Corticotropin-releasing factor receptor 2 antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human HeLa cell lysate with Corticotropin-releasing factor receptor 2 antibody. Predicted molecular weight ~48 kDa.
4 / 4
Flow cytometry testing of human MDA-MB-231 cells with Corticotropin-releasing factor receptor 2 antibody; Blue=isotype control, Green= Corticotropin-releasing factor receptor 2 antibody.

IHC testing of FFPE mouse brain tissue with Corticotropin-releasing factor receptor 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Corticotropin-releasing factor receptor 2 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human HeLa cell lysate with Corticotropin-releasing factor receptor 2 antibody. Predicted molecular weight ~48 kDa.
Flow cytometry testing of human MDA-MB-231 cells with Corticotropin-releasing factor receptor 2 antibody; Blue=isotype control, Green= Corticotropin-releasing factor receptor 2 antibody.

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Corticotropin-releasing factor receptor 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CRHR2 is a receptor for corticotropin releasing factor.It Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 243-272 from the human protein was used as the immunogen for the Corticotropin-releasing factor receptor 2 antibody.
Limitation:
This Corticotropin-releasing factor receptor 2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q13324