IL2RG Antibody / IL2 Receptor gamma

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NSJ Bioreagents
Product Code: NSJ-F54473
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54473-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54473-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the IL2 Receptor gamma antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) spleen, 2) Jurkat, 3) Daudi, 4) KG1, 5) MOLT4 and 6) HL60 cell lysate with IL2 Receptor gamma antibody. Expected molecular weight: 42-69 kDa depending on glycosylation level.
2 / 3
Immunofluorescent staining of FFPE human spleen tissue with IL2 Receptor gamma antibody (green) and DAPI nuclear stain (blue).
3 / 3
Flow cytometry testing of human HL60 cells with IL2 Receptor gamma antibody; Blue=isotype control, Green= IL2 Receptor gamma antibody.
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Western blot testing of human 1) spleen, 2) Jurkat, 3) Daudi, 4) KG1, 5) MOLT4 and 6) HL60 cell lysate with IL2 Receptor gamma antibody. Expected molecular weight: 42-69 kDa depending on glycosylation level.
Immunofluorescent staining of FFPE human spleen tissue with IL2 Receptor gamma antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human HL60 cells with IL2 Receptor gamma antibody; Blue=isotype control, Green= IL2 Receptor gamma antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the IL2 Receptor gamma antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
IL2RG is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 76-101 from the human protein was used as the immunogen for the IL2 Receptor gamma antibody.
Limitation:
This IL2 Receptor gamma antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P31785