STRADA Antibody / LYK5

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NSJ Bioreagents
Product Code: NSJ-F54440
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54440-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54440-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the LYK5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) U-2 OS, 2) SH-SY5Y and 3) MOLT4 cell lysate with LYK5 antibody. Predicted molecular weight ~48 kDa.
2 / 5
Western blot testing of human A549 cell lysate with LYK5 antibody. Predicted molecular weight ~48 kDa.
3 / 5
Western blot testing of mouse brain lysate with LYK5 antibody. Predicted molecular weight ~48 kDa.
4 / 5
Immunofluorescent staining of fixed and permeabilized human A549 cells with LYK5 antibody (green) and DAPI nuclear stain (blue).
5 / 5
Flow cytometry testing of fixed and permeabilized human A549 cells with LYK5 antibody; Blue=isotype control, Green= LYK5 antibody.
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Western blot testing of human 1) U-2 OS, 2) SH-SY5Y and 3) MOLT4 cell lysate with LYK5 antibody. Predicted molecular weight ~48 kDa.
Western blot testing of human A549 cell lysate with LYK5 antibody. Predicted molecular weight ~48 kDa.
Western blot testing of mouse brain lysate with LYK5 antibody. Predicted molecular weight ~48 kDa.
Immunofluorescent staining of fixed and permeabilized human A549 cells with LYK5 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of fixed and permeabilized human A549 cells with LYK5 antibody; Blue=isotype control, Green= LYK5 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the LYK5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
LYK5, also STE20-related Adaptor Protein (STRAD) are members of STE-20 like kinase family that are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. STRAD-LKB1 interaction results in phosphorylation of STRAD and enhanced autophosphorylation of LKB1.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 12-41 from the human protein was used as the immunogen for the LYK5 antibody.
Limitation:
This LYK5 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q7RTN6