ATP5O Antibody

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NSJ Bioreagents
Product Code: NSJ-F54457
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54457-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54457-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ATP5O antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human skeletal muscle tissue with ATP5O antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of FFPE human skeletal muscle with ATP5O antibody.
3 / 4
Western blot testing of human HEK293 cell lysate with ATP5O antibody. Predicted molecular weight ~23 kDa.
4 / 4
Flow cytometry testing of human HEK293 cells with ATP5O antibody; Blue=isotype control, Green= ATP5O antibody.
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IHC testing of FFPE human skeletal muscle tissue with ATP5O antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of FFPE human skeletal muscle with ATP5O antibody.
Western blot testing of human HEK293 cell lysate with ATP5O antibody. Predicted molecular weight ~23 kDa.
Flow cytometry testing of human HEK293 cells with ATP5O antibody; Blue=isotype control, Green= ATP5O antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the ATP5O antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 38-64 from the human protein was used as the immunogen for the ATP5O antibody.
Limitation:
This ATP5O antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P48047