ALKBH5 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54722
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54722-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54722-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ALKBH5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human HEK293 cell lysate with ALKBH5 antibody. Predicted molecular weight: ~52 kDa.
2 / 4
Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with ALKBH5 antibody. Predicted molecular weight: ~52 kDa.
3 / 4
IHC testing of FFPE human testis tissue with ALKBH5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with ALKBH5 antibody; Blue=isotype control, Green= ALKBH5 antibody.
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Western blot testing of human HEK293 cell lysate with ALKBH5 antibody. Predicted molecular weight: ~52 kDa.
Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with ALKBH5 antibody. Predicted molecular weight: ~52 kDa.
IHC testing of FFPE human testis tissue with ALKBH5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with ALKBH5 antibody; Blue=isotype control, Green= ALKBH5 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the ALKBH5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 302-330 from the human protein was used as the immunogen for the ALKBH5 antibody.
Limitation:
This ALKBH5 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q6P6C2