ASMT Antibody / Acetylserotonin O-methyltransferase

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NSJ Bioreagents
Product Code: NSJ-F54808
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54808-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54808-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ASMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human Y79 cell lysate with ASMT antibody. Predicted molecular weight ~38 kDa.
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Western blot testing of human HEK293 cell lysate with ASMT antibody. Predicted molecular weight ~38 kDa.
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IHC testing of FFPE human brain tissue with ASMT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HEK293 cells with ASMT antibody; Blue=isotype control, Green= ASMT antibody.
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Western blot testing of human Y79 cell lysate with ASMT antibody. Predicted molecular weight ~38 kDa.
Western blot testing of human HEK293 cell lysate with ASMT antibody. Predicted molecular weight ~38 kDa.
IHC testing of FFPE human brain tissue with ASMT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with ASMT antibody; Blue=isotype control, Green= ASMT antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50
Application Note:
The stated application concentrations are suggested starting points. Titration of the ASMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ASMT belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 212-241 from the human protein was used as the immunogen for the ASMT antibody.
Limitation:
This ASMT antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P46597