Calponin 1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54886
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54886-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54886-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Calponin 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Flow cytometry testing of human NCI-H292 cells with Calponin 1 antibody; Blue=isotype control, Green= Calponin 1 antibody.
2 / 4
IHC testing of FFPE human lung carcinoma tissue tissue with Calponin 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human NCI-H460 cell lysate with Calponin 1 antibody. Predicted molecular weight ~33 kDa.
4 / 4
Western blot testing of human 1) HeLa and 2) MDA-MB-453 cell lysate with Calponin 1 antibody. Predicted molecular weight ~33 kDa.
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Flow cytometry testing of human NCI-H292 cells with Calponin 1 antibody; Blue=isotype control, Green= Calponin 1 antibody.
IHC testing of FFPE human lung carcinoma tissue tissue with Calponin 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human NCI-H460 cell lysate with Calponin 1 antibody. Predicted molecular weight ~33 kDa.
Western blot testing of human 1) HeLa and 2) MDA-MB-453 cell lysate with Calponin 1 antibody. Predicted molecular weight ~33 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50
Application Note:
The stated application concentrations are suggested starting points. Titration of the Calponin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 10-39 from the human protein was used as the immunogen for the Calponin 1 antibody.
Limitation:
This Calponin 1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P51911