CD168 Antibody / HMMR

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NSJ Bioreagents
Product Code: NSJ-F54611
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54611-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54611-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CD168 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human breast carcinoma tissue with CD168 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human 1) K562, 2) LNCaP and 3) HL60 cell lysate with CD168 antibody. Expected molecular weight ~72 kDa (cell surface form) and 85-95 kDa (intracellular form).
3 / 4
Western blot testing of human Ramos cell lysate with CD168 antibody. Expected molecular weight ~72 kDa (cell surface form) and 85-95 kDa (intracellular form).
4 / 4
Flow cytometry testing of human Ramos cells with CD168 antibody; Blue=isotype control, Green= CD168 antibody.
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IHC testing of FFPE human breast carcinoma tissue with CD168 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) K562, 2) LNCaP and 3) HL60 cell lysate with CD168 antibody. Expected molecular weight ~72 kDa (cell surface form) and 85-95 kDa (intracellular form).
Western blot testing of human Ramos cell lysate with CD168 antibody. Expected molecular weight ~72 kDa (cell surface form) and 85-95 kDa (intracellular form).
Flow cytometry testing of human Ramos cells with CD168 antibody; Blue=isotype control, Green= CD168 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the CD168 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 668-697 from the human protein was used as the immunogen for the CD168 antibody.
Limitation:
This CD168 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O75330