DcR3 Antibody / TNFRSF6B

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NSJ Bioreagents
Product Code: NSJ-F54535
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54535-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54535-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the DcR3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human HEK293 cell lysate with DcR3 antibody. Predicted molecular weight ~33 kDa.
2 / 4
Western blot testing of human 1) HeLa, 2) HCT116, 3) PC-3, 4) SW480 and 5) HUVEC cell lysate with DcR3 antibody. Predicted molecular weight ~33 kDa.
3 / 4
IHC testing of FFPE human colon carcinoma tissue with DcR3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HEK293 cells with DcR3 antibody; Blue=isotype control, Green= DcR3 antibody.
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Western blot testing of human HEK293 cell lysate with DcR3 antibody. Predicted molecular weight ~33 kDa.
Western blot testing of human 1) HeLa, 2) HCT116, 3) PC-3, 4) SW480 and 5) HUVEC cell lysate with DcR3 antibody. Predicted molecular weight ~33 kDa.
IHC testing of FFPE human colon carcinoma tissue with DcR3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with DcR3 antibody; Blue=isotype control, Green= DcR3 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the DcR3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene belongs to the tumor necrosis factor receptor superfamily. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death. It acts as a decoy receptor that competes with death receptors for ligand binding. Overexpression of this gene has been noted in gastrointestinal tract tumors, and it is located in a gene-rich cluster on chromosome 20, with other potentially tumor-related genes. Two transcript variants encoding the same isoform, but differing in the 5' UTR, have been observed for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 22-48 from the human protein was used as the immunogen for the DcR3 antibody.
Limitation:
This DcR3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O95407