EIF4A2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54470
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54470-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54470-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the EIF4A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human lung carcinoma tissue with EIF4A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Western blot testing of human MCF7 cell lysate with EIF4A2 antibody. Predicted molecular weight ~46 kDa.
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Western blot testing of human K562 cell lysate with EIF4A2 antibody. Predicted molecular weight ~46 kDa.
4 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with EIF4A2 antibody.
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Flow cytometry testing of human K562 cells with EIF4A2 antibody; Blue=isotype control, Green= EIF4A2 antibody.
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IHC testing of FFPE human lung carcinoma tissue with EIF4A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human MCF7 cell lysate with EIF4A2 antibody. Predicted molecular weight ~46 kDa.
Western blot testing of human K562 cell lysate with EIF4A2 antibody. Predicted molecular weight ~46 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with EIF4A2 antibody.
Flow cytometry testing of human K562 cells with EIF4A2 antibody; Blue=isotype control, Green= EIF4A2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the EIF4A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation (referenced from OMIM).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 333-360 from the human protein was used as the immunogen for the EIF4A2 antibody.
Limitation:
This EIF4A2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q14240