FHL1 Antibody / SLIM1

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NSJ Bioreagents
Product Code: NSJ-F54890
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54890-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54890-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the FHL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human skeletal muscle tissue with FHL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with FHL1 antibody.
3 / 4
Western blot testing of mouse NIH 3T3 cell lysate with FHL1 antibody. Predicted molecular weight ~36 kDa.
4 / 4
Flow cytometry testing of human WiDr cells with FHL1 antibody; Blue=isotype control, Green= FHL1 antibody.
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IHC testing of FFPE human skeletal muscle tissue with FHL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with FHL1 antibody.
Western blot testing of mouse NIH 3T3 cell lysate with FHL1 antibody. Predicted molecular weight ~36 kDa.
Flow cytometry testing of human WiDr cells with FHL1 antibody; Blue=isotype control, Green= FHL1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the FHL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FHL1 is a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 214-240 from the human protein was used as the immunogen for the FHL1 antibody.
Limitation:
This FHL1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q13642