Folate Receptor alpha Antibody / FOLR1

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NSJ Bioreagents
Product Code: NSJ-F54555
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54555-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54555-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Folate Receptor alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human kidney tissue with Folate Receptor alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) 293T and 2) A549 cell lysate with Folate Receptor alpha antibody. Predicted molecular weight ~30 kDa but may be observed at higher molecular weights due to glycosylation.
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Western blot testing of human HeLa cell lysate with Folate Receptor alpha antibody. Predicted molecular weight ~30 kDa but may be observed at higher molecular weights due to glycosylation.
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Flow cytometry testing of fixed and permeabilized human HeLa cells with Folate Receptor alpha antibody; Blue=isotype control, Green= Folate Receptor alpha antibody.
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IHC testing of FFPE human kidney tissue with Folate Receptor alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) 293T and 2) A549 cell lysate with Folate Receptor alpha antibody. Predicted molecular weight ~30 kDa but may be observed at higher molecular weights due to glycosylation.
Western blot testing of human HeLa cell lysate with Folate Receptor alpha antibody. Predicted molecular weight ~30 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of fixed and permeabilized human HeLa cells with Folate Receptor alpha antibody; Blue=isotype control, Green= Folate Receptor alpha antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Folate Receptor alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 33-68 from the human protein was used as the immunogen for the Folate Receptor alpha antibody.
Limitation:
This Folate Receptor alpha antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P15328