GADD45A Antibody

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NSJ Bioreagents
Product Code: NSJ-F54774
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54774-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54774-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the GADD45A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human tonsil tissue with GADD45A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of Y79 cell lysate with GADD45A antibody. Expected molecular weight: 14-18 kDa (monomer) and 28-36 kDa (dimer).
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with GADD45A antibody. Expected molecular weight: 14-18 kDa (monomer) and 28-36 kDa (dimer).
4 / 4
Flow cytometry testing of human K562 cells with GADD45A antibody; Blue=isotype control, Green= GADD45A antibody.
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IHC testing of FFPE human tonsil tissue with GADD45A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of Y79 cell lysate with GADD45A antibody. Expected molecular weight: 14-18 kDa (monomer) and 28-36 kDa (dimer).
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with GADD45A antibody. Expected molecular weight: 14-18 kDa (monomer) and 28-36 kDa (dimer).
Flow cytometry testing of human K562 cells with GADD45A antibody; Blue=isotype control, Green= GADD45A antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the GADD45A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for the GADD45A antibody.
Limitation:
This GADD45A antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P24522