GSTO1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54405
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54405-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54405-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the GSTO1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human skeletal muscle tissue with GSTO1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Western blot testing of human HepG2 cell lysate with GSTO1 antibody. Predicted molecular weight: ~28 kDa.
3 / 5
Western blot testing of human NCI-H460 cell lysate with GSTO1 antibody. Predicted molecular weight: ~28 kDa.
4 / 5
Western blot testing of 1) non-transfected and 2) transfected HEK293 cell lysate with GSTO1 antibody.
5 / 5
Flow cytometry testing of human NCI-H292 cells with GSTO1 antibody; Blue=isotype control, Green= GSTO1 antibody.
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IHC testing of FFPE human skeletal muscle tissue with GSTO1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HepG2 cell lysate with GSTO1 antibody. Predicted molecular weight: ~28 kDa.
Western blot testing of human NCI-H460 cell lysate with GSTO1 antibody. Predicted molecular weight: ~28 kDa.
Western blot testing of 1) non-transfected and 2) transfected HEK293 cell lysate with GSTO1 antibody.
Flow cytometry testing of human NCI-H292 cells with GSTO1 antibody; Blue=isotype control, Green= GSTO1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the GSTO1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
GSTO1 is a member of the theta class glutathione S-transferase-like (GSTTL) protein family. In mouse, this protein acts as a small stress response protein, likely involved in cellular redox homeostasis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 126-155 from the human protein was used as the immunogen for the GSTO1 antibody.
Limitation:
This GSTO1 antibody is available for research use only.
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
P78417