HBE1 Antibody / Hemoglobin subunit epsilon

NSJ Bioreagents
Product Code: NSJ-F54383
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54383-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54383-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HBE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) placenta and 2) K562 lysate with HBE1 antibody. Predicted molecular weight ~16 kDa.
2 / 5
Western blot testing of human NCCIT cell lysate with HBE1 antibody. Predicted molecular weight ~16 kDa.
3 / 5
Western blot testing of human 1) placenta, 2) kidney, 3) spleen and 4) liver lysate with HBE1 antibody. Predicted molecular weight ~16 kDa.
4 / 5
IHC testing of FFPE human placenta tissue with HBE1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of fixed and permeabilized human K562 cells with HBE1 antibody; Blue=isotype control, Green= HBE1 antibody.

Western blot testing of human 1) placenta and 2) K562 lysate with HBE1 antibody. Predicted molecular weight ~16 kDa.
Western blot testing of human NCCIT cell lysate with HBE1 antibody. Predicted molecular weight ~16 kDa.
Western blot testing of human 1) placenta, 2) kidney, 3) spleen and 4) liver lysate with HBE1 antibody. Predicted molecular weight ~16 kDa.
IHC testing of FFPE human placenta tissue with HBE1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human K562 cells with HBE1 antibody; Blue=isotype control, Green= HBE1 antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the HBE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3'
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 55-83 from the human protein was used as the immunogen for the HBE1 antibody.
Limitation:
This HBE1 antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P02100