HSPA7 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F54463
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54463-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54463-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HSPA7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human testis tissue with HSPA7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human lung adenocarcinoma tissue with HSPA7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of human HEK293 cell lysate with HSPA7 antibody. Predicted molecular weight ~40 kDa, commonly observed at ~70 kDa.
4 / 5
Western blot testing of human CCRF-CEM cell lysate with HSPA7 antibody. Predicted molecular weight ~40 kDa, commonly observed at ~70 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with HSPA7 antibody; Blue=isotype control, Green= HSPA7 antibody.
❮❯

IHC testing of FFPE human testis tissue with HSPA7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human lung adenocarcinoma tissue with HSPA7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HEK293 cell lysate with HSPA7 antibody. Predicted molecular weight ~40 kDa, commonly observed at ~70 kDa.
Western blot testing of human CCRF-CEM cell lysate with HSPA7 antibody. Predicted molecular weight ~40 kDa, commonly observed at ~70 kDa.
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with HSPA7 antibody; Blue=isotype control, Green= HSPA7 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the HSPA7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 169-196 from the human protein was used as the immunogen for the HSPA7 antibody.
Limitation:
This HSPA7 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P48741