Bradykinin Antibody / Kininogen

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NSJ Bioreagents
Product Code: NSJ-F54608
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54608-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54608-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the KNG1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human Ramos cell lysate with KNG1 antibody. Predicted molecular weight ~72 kDa.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with KNG1 antibody. Predicted molecular weight ~72 kDa.
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IHC testing of FFPE human breast carcinoma tissue with KNG1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human Ramos cells with KNG1 antibody; Blue=isotype control, Green= KNG1 antibody.
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Western blot testing of human Ramos cell lysate with KNG1 antibody. Predicted molecular weight ~72 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with KNG1 antibody. Predicted molecular weight ~72 kDa.
IHC testing of FFPE human breast carcinoma tissue with KNG1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human Ramos cells with KNG1 antibody; Blue=isotype control, Green= KNG1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the KNG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 138-166 from the human protein was used as the immunogen for the KNG1 antibody.
Limitation:
This KNG1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P01042