RBPJL Antibody / RBP-L

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NSJ Bioreagents
Product Code: NSJ-F54631
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54631-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54631-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the RBP-L antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human pancreas tissue with RBP-L antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human ThP-1 cell lysate with RBP-L antibody. Predicted molecular weight ~57 kDa.
3 / 4
Western blot testing of HUVEC lysate with RBP-L antibody. Predicted molecular weight ~57 kDa.
4 / 4
Flow cytometry testing of human Jurkat cells with RBP-L antibody; Blue=isotype control, Green= RBP-L antibody.
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IHC testing of FFPE human pancreas tissue with RBP-L antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human ThP-1 cell lysate with RBP-L antibody. Predicted molecular weight ~57 kDa.
Western blot testing of HUVEC lysate with RBP-L antibody. Predicted molecular weight ~57 kDa.
Flow cytometry testing of human Jurkat cells with RBP-L antibody; Blue=isotype control, Green= RBP-L antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the RBP-L antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
In mouse, recombining binding protein L (RBP-L) is a transcription factor that binds to DNA sequences almost identical to that bound by the Notch receptor signalling pathway transcription factor RBP-J. However, unlike RBP-J, RBP-L does not interact with Notch receptors. RBP-L has been shown to activate transcription in concert with Epstein-Barr virus nuclear antigen-2 (EBNA2). The protein encoded by this gene is similar in sequence to the mouse RPB-L protein and Drosophila suppressor of hairless protein.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 8-36 from the human protein was used as the immunogen for the RBP-L antibody.
Limitation:
This RBP-L antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9UBG7