RNF4 Antibody

NSJ Bioreagents
Product Code: NSJ-F54707
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54707-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54707-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the RNF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human heart tissue with RNF4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human kidney tissue lysate with RNF4 antibody. Expected molecular weight: 15-30 kDa (isoform 1).
3 / 4
Western blot testing of human LNCaP cell lysate with RNF4 antibody. Expected molecular weight: 15-30 kDa (isoform 1).
4 / 4
Flow cytometry testing of fixed and permeabilized human HepG2 cells with RNF4 antibody; Blue=isotype control, Green= RNF4 antibody.

IHC testing of FFPE human heart tissue with RNF4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human kidney tissue lysate with RNF4 antibody. Expected molecular weight: 15-30 kDa (isoform 1).
Western blot testing of human LNCaP cell lysate with RNF4 antibody. Expected molecular weight: 15-30 kDa (isoform 1).
Flow cytometry testing of fixed and permeabilized human HepG2 cells with RNF4 antibody; Blue=isotype control, Green= RNF4 antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the RNF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 95-123 from the human protein was used as the immunogen for the RNF4 antibody.
Limitation:
This RNF4 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P78317