Transaldolase Antibody / TALDO1

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NSJ Bioreagents
Product Code: NSJ-F54871
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54871-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54871-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Transaldolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Transaldolase antibody.
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Western blot testing of human A375 cell lysate with Transaldolase antibody. Predicted molecular weight ~38 kDa.
3 / 4
IHC testing of FFPE human lung carcinoma tissue with Transaldolase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human K562 cells with Transaldolase antibody; Blue=isotype control, Green= Transaldolase antibody.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Transaldolase antibody.
Western blot testing of human A375 cell lysate with Transaldolase antibody. Predicted molecular weight ~38 kDa.
IHC testing of FFPE human lung carcinoma tissue with Transaldolase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with Transaldolase antibody; Blue=isotype control, Green= Transaldolase antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the Transaldolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Transaldolase 1 is a key enzyme of the nonoxidative pentose phosphate pathway providing ribose-5-phosphate for nucleic acid synthesis and NADPH for lipid biosynthesis. This pathway can also maintain glutathione at a reduced state and thus protect sulfhydryl groups and cellular integrity from oxygen radicals.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 186-215 from the human protein was used as the immunogen for the Transaldolase antibody.
Limitation:
This Transaldolase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P37837