TUFM Antibody

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NSJ Bioreagents
Product Code: NSJ-F54793
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54793-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54793-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TUFM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human hepatocellular carcinoma tissue with TUFM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human 1) Ramos and 2) HeLa cell lysate with TUFM antibody. Predicted molecular weight ~50 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with TUFM antibody.
4 / 4
Flow cytometry testing of human HeLa cells with TUFM antibody; Blue=isotype control, Green= TUFM antibody.
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IHC testing of FFPE human hepatocellular carcinoma tissue with TUFM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) Ramos and 2) HeLa cell lysate with TUFM antibody. Predicted molecular weight ~50 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with TUFM antibody.
Flow cytometry testing of human HeLa cells with TUFM antibody; Blue=isotype control, Green= TUFM antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the TUFM antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TUFM is a protein which participates in protein translation in mitochondria. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 75-104 from the human protein was used as the immunogen for the TUFM antibody.
Limitation:
This TUFM antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P49411