Cyclophilin D Antibody

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NSJ Bioreagents
Product Code: NSJ-F54323
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54323-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54323-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Cyclophilin D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) K562, 2) HepG2, 3) Jurkat, 4) CCRF-CEM and 5) brain lysate with Cyclophilin D antibody. Predicted molecular weight: ~40 kDa
2 / 5
IHC testing of FFPE human brain tissue with Cyclophilin D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Cyclophilin D antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 5
Flow cytometry testing of fixed and permeabilized human HepG2 cells with Cyclophilin D antibody; Blue=isotype control, Green= Cyclophilin D antibody.
5 / 5
Flow cytometry testing of fixed and permeabilized human K562 cells with Cyclophilin D antibody; Blue=isotype control, Green= Cyclophilin D antibody.
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Western blot testing of human 1) K562, 2) HepG2, 3) Jurkat, 4) CCRF-CEM and 5) brain lysate with Cyclophilin D antibody. Predicted molecular weight: ~40 kDa
IHC testing of FFPE human brain tissue with Cyclophilin D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Cyclophilin D antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human HepG2 cells with Cyclophilin D antibody; Blue=isotype control, Green= Cyclophilin D antibody.
Flow cytometry testing of fixed and permeabilized human K562 cells with Cyclophilin D antibody; Blue=isotype control, Green= Cyclophilin D antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Cyclophilin D antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA- binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity. Involved in regulation of UV radiation-induced apoptosis. Promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large- cell lymphoma (ALK+ ALCL) cell lines. May be involved in hepatitis C virus (HCV) replication and release.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 336-370 from the human protein was used as the immunogen for the Cyclophilin D antibody.
Limitation:
This Cyclophilin D antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q08752