Integrin alpha 7 Antibody / ITGA7

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NSJ Bioreagents
Product Code: NSJ-F54396
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54396-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54396-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Integrin alpha 7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) HeLa, 2) A431 and 3) Jurkat cell lysate with Integrin alpha 7 antibody. Predicted molecular weight ~129 kDa.
2 / 4
IHC testing of FFPE human skeletal muscle tissue with Integrin alpha 7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Integrin alpha 7 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with Integrin alpha 7 antibody; Blue=isotype control, Green= Integrin alpha 7 antibody.
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Western blot testing of human 1) HeLa, 2) A431 and 3) Jurkat cell lysate with Integrin alpha 7 antibody. Predicted molecular weight ~129 kDa.
IHC testing of FFPE human skeletal muscle tissue with Integrin alpha 7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Integrin alpha 7 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with Integrin alpha 7 antibody; Blue=isotype control, Green= Integrin alpha 7 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Integrin alpha 7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 247-279 from the human protein was used as the immunogen for the Integrin alpha 7 antibody.
Limitation:
This Integrin alpha 7 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q13683