Rubicon Antibody / RUBCN / BARON

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NSJ Bioreagents
Product Code: NSJ-F54720
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54720-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54720-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Rubicon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) K562 and 2) HEK293 cell lysate with Rubicon antibody. Expected molecular weight ~130 kDa.
2 / 4
IHC testing of FFPE human lymph tissue with Rubicon antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with Rubicon antibody (green) and Hoechst 33342 nuclear stain (blue).
4 / 4
Flow cytometry testing of human MDA-231 cells with Rubicon antibody; Blue=isotype control, Green= Rubicon antibody.
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Western blot testing of human 1) K562 and 2) HEK293 cell lysate with Rubicon antibody. Expected molecular weight ~130 kDa.
IHC testing of FFPE human lymph tissue with Rubicon antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with Rubicon antibody (green) and Hoechst 33342 nuclear stain (blue).
Flow cytometry testing of human MDA-231 cells with Rubicon antibody; Blue=isotype control, Green= Rubicon antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Rubicon antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 241-271 from the human protein was used as the immunogen for the Rubicon antibody.
Limitation:
This Rubicon antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q92622