Brachyury Antibody / Protein T / TBXT

NSJ Bioreagents
Product Code: NSJ-F54735
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54735-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54735-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the Brachyury antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot testing of human 1) A431 and 2) NCI-H460 cell lysate with Brachyury antibody. Predicted molecular weight ~47 kDa.
2 / 3
Western blot testing of human MDA-MB-453 cell lysate with Brachyury antibody. Predicted molecular weight ~47 kDa.
3 / 3
Immunofluorescent staining of fixed and permeabilized human A549 cells with Brachyury antibody (green) and DAPI nuclear stain (blue).

Western blot testing of human 1) A431 and 2) NCI-H460 cell lysate with Brachyury antibody. Predicted molecular weight ~47 kDa.
Western blot testing of human MDA-MB-453 cell lysate with Brachyury antibody. Predicted molecular weight ~47 kDa.
Immunofluorescent staining of fixed and permeabilized human A549 cells with Brachyury antibody (green) and DAPI nuclear stain (blue).

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Brachyury antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by the TBXT gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 15-43 from the human protein was used as the immunogen for the Brachyury antibody.
Limitation:
This Brachyury antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O15178