Glycogen debranching enzyme Antibody / AGL

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NSJ Bioreagents
Product Code: NSJ-F54768
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54768-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54768-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the Glycogen debranching enzyme antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human skeletal muscle tissue lysate with Glycogen debranching enzyme antibody. Predicted molecular weight ~175 kDa.
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Western blot testing of human tissue lysate with Glycogen debranching enzyme antibody. Predicted molecular weight ~175 kDa. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan.
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Immunofluorescent staining of human HepG2 cells with Glycogen debranching enzyme antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
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Western blot testing of human skeletal muscle tissue lysate with Glycogen debranching enzyme antibody. Predicted molecular weight ~175 kDa.
Western blot testing of human tissue lysate with Glycogen debranching enzyme antibody. Predicted molecular weight ~175 kDa. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan.
Immunofluorescent staining of human HepG2 cells with Glycogen debranching enzyme antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Documents

Further Information

Application Details :
Western blot: 1:2000-1:8000, Immunofluorescence: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting points. Titration of the Glycogen debranching enzyme antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1479-1510 from the human protein was used as the immunogen for the Glycogen debranching enzyme antibody.
Limitation:
This Glycogen debranching enzyme antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P35573