Adiponectin Receptor 2 Antibody / ADIPOR2

NSJ Bioreagents
Product Code: NSJ-F54293
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54293-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54293-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the Adiponectin Receptor 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) U-251 and 2) U-2 OS cell lysate with Adiponectin Receptor 2 antibody. Predicted molecular weight ~44 kDa.
2 / 5
Western blot testing of human liver lysate with Adiponectin Receptor 2 antibody. Predicted molecular weight ~44 kDa.
3 / 5
Western blot testing of human HeLa cell lysate with Adiponectin Receptor 2 antibody. Predicted molecular weight ~44 kDa.
4 / 5
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Adiponectin Receptor 2 antibody (green) and DAPI nuclear stain (blue).
5 / 5
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with Adiponectin Receptor 2 antibody; Blue=isotype control, Green= Adiponectin Receptor 2 antibody.

Western blot testing of human 1) U-251 and 2) U-2 OS cell lysate with Adiponectin Receptor 2 antibody. Predicted molecular weight ~44 kDa.
Western blot testing of human liver lysate with Adiponectin Receptor 2 antibody. Predicted molecular weight ~44 kDa.
Western blot testing of human HeLa cell lysate with Adiponectin Receptor 2 antibody. Predicted molecular weight ~44 kDa.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Adiponectin Receptor 2 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with Adiponectin Receptor 2 antibody; Blue=isotype control, Green= Adiponectin Receptor 2 antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Adiponectin Receptor 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The adiponectin receptors ADIPOR1 and ADIPOR2 serve as receptors for globular and full-length adiponectin and mediate increased AMPK and PPAR-alpha ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 45-72 from the human protein were used as the immunogen for the Adiponectin Receptor 2 antibody.
Limitation:
This Adiponectin Receptor 2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q86V24