APRT Antibody

NSJ Bioreagents
Product Code: NSJ-F54787
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54787-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54787-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Adenine phosphoribosyltransferase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with Adenine phosphoribosyltransferase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of fixed and permeabilized human A549 cells with Adenine phosphoribosyltransferase antibody (green) and anti-Actin (red).
3 / 4
Western blot testing of human A2058 cell lysate with Adenine phosphoribosyltransferase antibody. Predicted molecular weight ~20 kDa.
4 / 4
Flow cytometry testing of human HepG2 cells with Adenine phosphoribosyltransferase antibody; Blue=isotype control, Green= Adenine phosphoribosyltransferase antibody.

IHC testing of FFPE human hepatocellular carcinoma tissue with Adenine phosphoribosyltransferase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human A549 cells with Adenine phosphoribosyltransferase antibody (green) and anti-Actin (red).
Western blot testing of human A2058 cell lysate with Adenine phosphoribosyltransferase antibody. Predicted molecular weight ~20 kDa.
Flow cytometry testing of human HepG2 cells with Adenine phosphoribosyltransferase antibody; Blue=isotype control, Green= Adenine phosphoribosyltransferase antibody.

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Adenine phosphoribosyltransferase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 12-40 from the human protein was used as the immunogen for the Adenine phosphoribosyltransferase antibody.
Limitation:
This Adenine phosphoribosyltransferase antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P07741