Inosine triphosphate pyrophosphatase Antibody / ITPA / ITPase

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NSJ Bioreagents
Product Code: NSJ-F54812
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54812-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54812-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Inosine triphosphate pyrophosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) A375, 2) HeLa, 3) HepG2, 4) mouse brain and 5) rat liver lysate with Inosine triphosphate pyrophosphatase antibody. Predicted molecular weight ~21 kDa.
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IHC testing of FFPE human lung carcinoma tissue with Inosine triphosphate pyrophosphatase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Immunofluorescent staining of human HeLa cells with Inosine triphosphate pyrophosphatase antibody (green) and anti-Actin (red).
4 / 4
Flow cytometry testing of human A375 cells with Inosine triphosphate pyrophosphatase antibody; Blue=isotype control, Green= Inosine triphosphate pyrophosphatase antibody.
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Western blot testing of human 1) A375, 2) HeLa, 3) HepG2, 4) mouse brain and 5) rat liver lysate with Inosine triphosphate pyrophosphatase antibody. Predicted molecular weight ~21 kDa.
IHC testing of FFPE human lung carcinoma tissue with Inosine triphosphate pyrophosphatase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Inosine triphosphate pyrophosphatase antibody (green) and anti-Actin (red).
Flow cytometry testing of human A375 cells with Inosine triphosphate pyrophosphatase antibody; Blue=isotype control, Green= Inosine triphosphate pyrophosphatase antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the Inosine triphosphate pyrophosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ITPA hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. The encoded protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency. Two transcript variants encoding two different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 24-51 from the human protein was used as the immunogen for the Inosine triphosphate pyrophosphatase antibody.
Limitation:
This Inosine triphosphate pyrophosphatase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9BY32