TSPYL6 Antibody

NSJ Bioreagents
Product Code: NSJ-F54591
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54591-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54591-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TSPYL6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human testis tissue with TSPYL6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HeLa cells with TSPYL6 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
Western blot testing of human 1) NCI-H460 and 2) HeLa cell lysate with TSPYL6 antibody. Predicted molecular weight ~46 kDa.
4 / 4
Flow cytometry testing of human HeLa cells with TSPYL6 antibody; Blue=isotype control, Green= TSPYL6 antibody.

IHC testing of FFPE human testis tissue with TSPYL6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with TSPYL6 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of human 1) NCI-H460 and 2) HeLa cell lysate with TSPYL6 antibody. Predicted molecular weight ~46 kDa.
Flow cytometry testing of human HeLa cells with TSPYL6 antibody; Blue=isotype control, Green= TSPYL6 antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the TSPYL6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Alternate names: Testis-specific Y-encoded-like protein 6; TSPY-like protein 6
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 206-235 from the human protein was used as the immunogen for the TSPYL6 antibody.
Limitation:
This TSPYL6 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q8N831