TYSY Antibody / Thymidylate Synthase

Contact us
NSJ Bioreagents
Product Code: NSJ-F54872
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54872-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54872-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TYSY antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human Jurkat cell lysate with TYSY antibody. Predicted molecular weight ~36 kDa.
2 / 4
IHC testing of FFPE human colon carcinoma tissue with TYSY antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human HeLa cells with TYSY antibody (green) and anti-Actin (red).
4 / 4
Flow cytometry testing of fixed and permeabilized human WiDr cells with TYSY antibody; Blue=isotype control, Green= TYSY antibody.
❮❯

Western blot testing of human Jurkat cell lysate with TYSY antibody. Predicted molecular weight ~36 kDa.
IHC testing of FFPE human colon carcinoma tissue with TYSY antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with TYSY antibody (green) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human WiDr cells with TYSY antibody; Blue=isotype control, Green= TYSY antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the TYSY antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 265-294 from the human protein was used as the immunogen for the TYSY antibody.
Limitation:
This TYSY antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P04818