cIAP2 Antibody / BIRC3

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NSJ Bioreagents
Product Code: NSJ-F54681
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54681-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54681-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the BIRC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with BIRC3 antibody. Predicted molecular weight ~68 kDa.
2 / 4
Western blot testing of human MDA-MB-435 cell lysate with BIRC3 antibody. Predicted molecular weight ~68 kDa.
3 / 4
Immunofluorescent staining of human MDA-MB-435 cells with BIRC3 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
IHC testing of FFPE human tonsil tissue with BIRC3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with BIRC3 antibody. Predicted molecular weight ~68 kDa.
Western blot testing of human MDA-MB-435 cell lysate with BIRC3 antibody. Predicted molecular weight ~68 kDa.
Immunofluorescent staining of human MDA-MB-435 cells with BIRC3 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human tonsil tissue with BIRC3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the BIRC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. The amino acid sequence predicts three baculovirus IAP repeat domains and a ring finger domain. Transcript variants encoding the same isoform have been identified. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 59-88 from the human protein was used as the immunogen for the BIRC3 antibody.
Limitation:
This BIRC3 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q13489