HDAC2 Antibody

NSJ Bioreagents
Product Code: NSJ-F54273
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54273-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54273-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HDAC2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 293 cell lysate with HDAC2 antibody. Predicted molecular weight: 55-60 kDa.
2 / 5
Western blot testing of mouse spleen lysate with HDAC2 antibody. Predicted molecular weight: 55-60 kDa.
3 / 5
IHC testing of FFPE human breast carcinoma tissue with HDAC2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
Immunofluorescent staining of fixed and permeabilized human HeLa cells with HDAC2 antibody (green) and anti-Actin (red).
5 / 5
Immunofluorescent staining of fixed and permeabilized human 293 cells with HDAC2 antibody (green) and anti-Actin (red).

Western blot testing of human 293 cell lysate with HDAC2 antibody. Predicted molecular weight: 55-60 kDa.
Western blot testing of mouse spleen lysate with HDAC2 antibody. Predicted molecular weight: 55-60 kDa.
IHC testing of FFPE human breast carcinoma tissue with HDAC2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with HDAC2 antibody (green) and anti-Actin (red).
Immunofluorescent staining of fixed and permeabilized human 293 cells with HDAC2 antibody (green) and anti-Actin (red).

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the HDAC2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 456-488 from the human protein were used as the immunogen for the HDAC2 antibody.
Limitation:
This HDAC2 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
Q92769