CNOT8 Antibody / CCR4-NOT transcription complex subunit 8

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NSJ Bioreagents
Product Code: NSJ-F54856
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54856-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54856-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CNOT8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human colon carcinoma tissue with CNOT8 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human U-251 cell lysate with CNOT8 antibody. Predicted molecular weight ~33 kDa.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with CNOT8 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of human CCRF-CEM cells with CNOT8 antibody; Blue=isotype control, Green= CNOT8 antibody.
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IHC testing of FFPE human colon carcinoma tissue with CNOT8 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human U-251 cell lysate with CNOT8 antibody. Predicted molecular weight ~33 kDa.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with CNOT8 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of human CCRF-CEM cells with CNOT8 antibody; Blue=isotype control, Green= CNOT8 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the CNOT8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 227-255 from the human protein was used as the immunogen for the CNOT8 antibody.
Limitation:
This CNOT8 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9UFF9