Carboxypeptidase M Antibody / CPM

NSJ Bioreagents
Product Code: NSJ-F54598
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54598-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54598-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CPM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human kidney tissue lysate with CPM antibody. Expected molecular weight: 51-65 kDa depending on glycosylation level.
2 / 4
Western blot testing of human 1) U-251 MG and 2) HEK293 cell lysate with CPM antibody. Expected molecular weight: 51-65 kDa depending on glycosylation level.
3 / 4
IHC testing of FFPE human adrenal tissue with CPM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
IHC testing of FFPE human kidney tissue with CPM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Western blot testing of human kidney tissue lysate with CPM antibody. Expected molecular weight: 51-65 kDa depending on glycosylation level.
Western blot testing of human 1) U-251 MG and 2) HEK293 cell lysate with CPM antibody. Expected molecular weight: 51-65 kDa depending on glycosylation level.
IHC testing of FFPE human adrenal tissue with CPM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with CPM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the CPM antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 212-241 from the human protein was used as the immunogen for the CPM antibody.
Limitation:
This CPM antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P14384